However, AI virus penetrates the cells through a receptor-mediated endocytosis mechanism (Swayne and King, 2003). As many studies have discussed the in?vitro antiviral activity of EOs against avian viruses after contact between Ferrostatin-1 (Fer-1) viruses and EOs for certain periods of time at room heat (Barbour et?al., 2010), here we designed PRV and TTT to study BMP2B the antiviral effect of EOs Ferrostatin-1 (Fer-1) in?vivo (in chickens) at chicken’s body temperature (41.5C) either before (PRV) or after vNDv challenge (TTT). VC (8.63??0.65 and 10 log 3.29??0.72, respectively). However, the EOs combination (VTC) (1952??28.82) did not significantly ( 0.05) improve growth performance compared with the nontreated birds (NC and VC) (1970??19.56 and 1904??38.66). EOs showed an antiviral effect on vNDv in?vivo (in chickens) like a preventive measure (PRV) as well while some therapeutic effect (TTT) through decreasing the viral dropping titres (loNC0), mortality rate, and severity of clinical indicators and postmortem lesions, in addition to serum malondialdhyde level. Concerning the additional viruses, the EOs combination did not improve the immune response to the AI and IB vaccines but significantly ( 0.05) increased the ELISA antibody titre for IBD computer virus in the 28th D of age (2,108??341.05). The analyzed EOs combination showed an immune-stimulating response to Ferrostatin-1 (Fer-1) ND and IBD vaccines, antiviral effect against ND computer virus, especially if given before the challenge; however, it did not possess a growth-promoting effect. computer virus of family (Cox et?al., 2000), while the etiology of IBD or Gumboro disease is the IBD computer virus that is a Ferrostatin-1 (Fer-1) member of genus of the family (Delmas et?al., 2019). IB computer virus is belonging to genus of family (Enjuanes et?al., 2000), while the cause of ND is definitely ND computer virus, belonging to genus of family (Hines and Miller, 2012). All these viruses possess RNA genome enclosed within either enveloped (AI, IB, and ND viruses) or nonenveloped (IBD computer virus) capsid. Their outbreaks still happen in the field, although several live, inactivated, or recombinant vaccines are commercially available, and different vaccination programs are applied for each disease (Swayne and King, 2003). Many factors are related to this problem, and one of them is the immune suppression or an immature immune system (Skowronski et?al., 2008). Moreover, if a computer virus is definitely endemic in a region, its elimination is definitely difficult, so additional methods are required to combat these viruses along with vaccination. Recently, there has been increased desire for the use of natural antimicrobial providers, and the use of EOs in combination as a strategy to control many pathogens (Gutierrez et?al., 2008). Over 3,000 EOs are known, of which approximately 300 are commercially important and used in the flavour and perfume industries (Vehicle de Braak and Leijten, 1999, Baghban-Kanani et?al., 2019). Essential oils are produced by medicinal vegetation, and their effects vary based on the component, which may possess antibacterial (Sokovic et?al., 2010, Qorbanpour et?al., 2018), antiviral (Koch et?al., 2008, Gado et?al., 2019), anticoccidial (Mohiti-Asli and Ghanaatparast-Rashti, 2015), antifungal (Pinto et?al., 2006), antioxidant (Nunez et?al., 2007), or growth-promoting effects (Abd El-Hack et?al., 2016a, Abd El-Hack et?al., 2016b, Abd El-Hack et?al., 2016c, Abd El-Hack et?al., 2018). However, Reichlinga et?al. (2009) concluded in their review the most tested essential oils exhibited a definite antiviral activity on enveloped DNA and RNA viruses, while they did not impact the nonenveloped viruses. In addition, you will find recent studies showing that EOs can improve the cellular and humoral immune responses of chickens (Lee et?al., 2011, Roofchaee et?al., 2011). We hypothesized that EOs combination stimulates immune response to different viral vaccines used in broiler flocks, promotes excess weight gaining, and can be used to the face the viral outbreaks such as ND computer virus. The objectives of this study included 1) evaluation of EOs’.