Primary cell culture was conducted as previously described30,57
Primary cell culture was conducted as previously described30,57. splicing variant (TFEB-S) lacking HLH and LZ regions of TFEB-L (Fig.?1A). To determine how the small TFEB was derived from the gene, we sequenced the small variant cloned into pHM6. Compared to the gene, the small CGP 36742 splicing variant was missing exon 8 (Fig.?1B). In addition,…