Biological), ATR (Santa Cruz), and mTOR (Cell Signaling) according to the manufacturer’s instructions
Biological), ATR (Santa Cruz), and mTOR (Cell Signaling) according to the manufacturer’s instructions. Flow Cytometric Quantification of BrdUrd Incorporation, Phospho-H2AX, and Phospho-histone H3G0-enriched cells were harvested 16-19 h after serum stimulation and fixed in 70% EtOH prior to staining. of ATR pathway failure. These results imply that ATR-dependent fork stabilization and H2AX/ATM/DNA-PKcs-dependent restart pathways cooperatively…