DLK proteins precipitated with DLK antibody was aliquoted for DLK kinase activity assays and Traditional western blotting
DLK proteins precipitated with DLK antibody was aliquoted for DLK kinase activity assays and Traditional western blotting. T659 and S584. Blocking PI3K/Akt signaling with LY-294002 improved DLK kinase activity significantly. We discovered that Akt interacts with and phosphorylates DLK. Mutations of DLK amino acidity residues at putative Akt phosphorylation sites (S584A, T659A, or S584A and…