For human cell lines infected with lentiviral shRNA constructs, 24 hours following spinoculation 3?g/mL puromycin (Sigma-Aldrich) was added for 48 hours to select for successfully transduced cells prior to further manipulation
For human cell lines infected with lentiviral shRNA constructs, 24 hours following spinoculation 3?g/mL puromycin (Sigma-Aldrich) was added for 48 hours to select for successfully transduced cells prior to further manipulation. bioinformatics, we find that FOXC1 and RUNX1 interact through Forkhead and Runt domains, respectively, and co-occupy primed and active enhancers distributed close to differentiation…