Second harmonic signals removed.(MP4) ppat.1005881.s015.mp4 (960K) GUID:?6EA9C689-7399-49DF-B431-1C145AE38301 S15 Movie: Video collected at day 9 at higher resolution (512p) for a closer, more detailed view of CD8 T cell behavior just after viral clearance. trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended focus videos, taken from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s003.mp4 (4.2M) GUID:?42A7DCD9-1CF7-49BD-A2BD-A60B19338BF6 S3 Movie: Extended focus videos of day 7 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended focus videos, taken from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s004.mp4 (2.7M) GUID:?DB71812D-3198-43CF-A908-DC57F381DBCD S4 Movie: Extended focus videos of day 8 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended Ansatrienin A focus videos, taken from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s005.mp4 (2.9M) GUID:?08993F05-2F3B-49B0-8B6B-BBBB54EAD012 S5 Movie: Extended focus videos of day 9 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended focus videos, taken from a point of Ansatrienin A view the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s006.mp4 (4.7M) GUID:?B4A2EF23-BC2D-46B6-8799-D182868D3C83 S6 Movie: Extended focus videos of day 10 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples Rabbit Polyclonal to OR5K1 of the extended focus videos, taken from a point of view inside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s007.mp4 (2.0M) GUID:?37819C40-0CBA-4F49-94B1-FCE830A3DD3D S7 Movie: Extended focus videos of day 14 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. The movies show examples of the extended focus videos, taken from a point of view outside the trachea peering through, used to generate the motility parameters for days 6C10 after infection.(MP4) ppat.1005881.s008.mp4 (417K) GUID:?CDBB5ADF-DF68-4779-8A8A-958C2D1F7B94 S8 Movie: 3D animation of day 6 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. Rotating 3D images were reconstructed from the extended focus videos to show the lateral point of view of the tissue to illustrate depth in the localization of T cells (green) relative to the outer collagen sheath (white).(MP4) ppat.1005881.s009.mp4 (12M) GUID:?95653F53-F469-43CD-8332-C49387084924 S9 Movie: 3D animation of day 7 CD8 T cell motility in the flu Ansatrienin A infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. Rotating 3D images were reconstructed from the extended focus videos to show the lateral point of view of the tissue to illustrate depth in the localization of T cells (green) relative to the outer collagen sheath (white).(MP4) ppat.1005881.s010.mp4 (6.4M) GUID:?E43E9BC6-1EF1-46C6-90F8-F9EE62AD124A S10 Movie: 3D animation of day 8 CD8 T cell motility in the flu infected trachea. Adoptively transferred OT-I-GFP CD8+ T cells (green) are imaged by multiphoton intravital microscopy after infection with influenza X31-OVA-I as described in materials and methods. Rotating 3D images were reconstructed from the extended focus videos to show the lateral point of.